Thanks for your replies gents.
Canute Rex – In hand, the mark looked exactly like solder as you observed. I followed your suggestions to sand, and then oxidize with white vinegar. The mark is now gone. Whatever it was, it seems to have been a surface phenomenon, and not an actual inclusion. Whew! If it had been an inclusion, I would have probably replaced the barrel. I do not have a clue what may have caused it, but I am much relieved.
kudu – Correct, the groove is for the front lock bolt. I do not finish the bottoms of my barrels, so the scratches are the history of the barrel being made, plus whatever scratches I added during the build. Someone once asked Jerry Huddleston if he polished the bottom flats of his barrels. He replied “No, and I don’t polish the bottoms of my shoes either”.
T*O*F – This is a Bobby Hoyt barrel. I believe it is 12L14. I hope you can get rid of your inclusions as easily as I got rid of mine.
Lightly sanded with 220.
Oxidized with white vinegar