I always index the sprues straight up on both smoothbore and my rifle loading. Yes - it makes a difference in accuracy as shown by smylee grouch.
The method I use to remove the sprues on my Tanner moulds, leaves no sprue to index, but a smooth rounded surface - those are not indexed
in my rifle or smoothbore. One of the postal matches I won, was with non-indexed sprue-less balls in the rifle, but then it was shot at 25 yards and this
likely made no consequence.
Balls with sprues when shot in a smoothbore, will at some point of the ball's travel (likely when sub-sonic), cause the ball to take on a spin & veer off
target (point of aim). Thus, balls with sprues, if loaded straight up and patched in a smoothbore, if more powder is used to increase the velocity, the range
at which the ball takes on that spin will be extended.
For example, I've witnessed on several occasions, one of the fellow's launched round ball at a 92 yard gong, in the last few yards (it looks) veer off to one side
or the other or down and miss the gong by 3', yet before they veered out of line, were traveling straight to the target in an arc. I have only observed a couple
launched by Taylor, but they have always traveled straight to the gong. The only difference in loading, is the first fellow used 65gr. 2F in his 20 bore and Taylor
uses 85gr. 2F.
I hit more of the longer range gongs with my smoothbore now that I use patched round balls that have no sprues. I also used 85gr. of powder in the past, but have
increased my charge to 90gr. 2F for trail walks due to the short barrel on my gun.